Bismark report for: /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/CF02-CM02-Zygote_R1_001.fastp-trim.fq.gz and /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/CF02-CM02-Zygote_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/kdurkin1/kathleen-coral/project/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 206045849 Number of paired-end alignments with a unique best hit: 27528519 Mapping efficiency: 13.4% Sequence pairs with no alignments under any condition: 167768755 Sequence pairs did not map uniquely: 10748575 Sequence pairs which were discarded because genomic sequence could not be extracted: 43 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 8331198 ((converted) top strand) GA/CT/CT: 5401679 (complementary to (converted) top strand) GA/CT/GA: 5415702 (complementary to (converted) bottom strand) CT/GA/GA: 8379897 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 560662988 Total methylated C's in CpG context: 13245595 Total methylated C's in CHG context: 4402157 Total methylated C's in CHH context: 28851821 Total methylated C's in Unknown context: 583161 Total unmethylated C's in CpG context: 50988738 Total unmethylated C's in CHG context: 92569772 Total unmethylated C's in CHH context: 370604905 Total unmethylated C's in Unknown context: 3089401 C methylated in CpG context: 20.6% C methylated in CHG context: 4.5% C methylated in CHH context: 7.2% C methylated in Unknown context (CN or CHN): 15.9% Bismark completed in 0d 10h 47m 23s