Bismark report for: /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/CF01-CM02-Larvae_R1_001.fastp-trim.fq.gz and /gscratch/srlab/kdurkin1/kathleen-coral/project/data/trimmed-WGBS-reads/CF01-CM02-Larvae_R2_001.fastp-trim.fq.gz (version: v0.24.2) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/kdurkin1/kathleen-coral/project/data/Cvirginica_v300/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 133403240 Number of paired-end alignments with a unique best hit: 46656964 Mapping efficiency: 35.0% Sequence pairs with no alignments under any condition: 67755480 Sequence pairs did not map uniquely: 18990796 Sequence pairs which were discarded because genomic sequence could not be extracted: 67 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 11335548 ((converted) top strand) GA/CT/CT: 11977584 (complementary to (converted) top strand) GA/CT/GA: 11985108 (complementary to (converted) bottom strand) CT/GA/GA: 11358657 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 1511988505 Total methylated C's in CpG context: 42901638 Total methylated C's in CHG context: 6275390 Total methylated C's in CHH context: 27139249 Total methylated C's in Unknown context: 1122139 Total unmethylated C's in CpG context: 190236750 Total unmethylated C's in CHG context: 320201916 Total unmethylated C's in CHH context: 925233562 Total unmethylated C's in Unknown context: 5776506 C methylated in CpG context: 18.4% C methylated in CHG context: 1.9% C methylated in CHH context: 2.8% C methylated in Unknown context (CN or CHN): 16.3% Bismark completed in 0d 7h 56m 27s