Completed re-extraction of the following samples in multiples of 4:
USNM 14366
USNM 19054
USNM 52295
USNM 1180630
USNM 50603
See full details in July 17 post.
Then used Zymo Cleanup Kit to clean and concentrate extractions, following the Zymo Cleanup Protocol found in my July 12 post, with the following modifications:
For each specimen, to concentrate 4 100uL extractions into a single cleaned extraction, during steps 2 & 3 of the protocol (adding sample to spin column and centrifuging), performed several iterations for each specimen. The spin column is to small to hold the full volume of 4 100uL DNA samples and the corresponding 2000uL of ChIP Binding Buffer. Instead, added 5 volumes of ChIP Binding Buffer to each extraction individually. Then added 1000uL of sample DNA + ChIP Buffer to spin column at a time, then centrifuged, discarded flow through, and repeated until all extracted DNA of a given specimen had been bound to the spin column. I then proceeded as normal, eluting in a final volume of 50uL of heated Elution Buffer.