Quantified DNA concentration of my 5 re-extractions using Qubit flourometer. Followed Qubit Protocol found in my July 14 post.
Standard #1: 37.13
Standard #2: 14435.49
| Catalog Number | Qubit Label | Concentration (ng/uL) |
|---|---|---|
| USNM 14366 | C01 | 0.372 |
| USNM 19054 | C02 | 3.57 |
| USNM 52295 | C03 | 9.02 |
| USNM 1180630 | C04 | 1.78 |
| USNM 50603 | C05 | 14.9 |
Notes:
Ok this isn’t terrible! The 50603 concentrated re-extraction has >10ng/uL, which is enough to meet the recommended 400ng minimum for MinION. USNM52295 is almost at this threshold, and if I can combine it with my first extraction of this specimen ([ ] = 7.08ng/uL), e.g. during bead cleanup, we’ll have enough of that one too!
That would bring me to my desired minimum number of samples (n=5) for the >100yo specimens!
Now I need to run gels on everything to check fragment size. I should also do a Nanodrop at some point before library prep to check for contaminants.