Finishing extractions

Completed E. tourneforti extractions

Qiagen DNEasy Extraction Protocol (after digestion)

2. Add 200uL Buffer AL. Mix thoroughly by vortexing, and spin down the sides.
3. Add 200ul 95% ethanol. Mix thoroughly by vortexing, and spin down the sides.
4. Pipet mixture onto a labelled DNEasy Mini Spin Column placed in a 2mL collection tube. Centrifuge @ >= 8,000 RPM for 1 min. Discard the flow through and collection tube.
5. Place column in a new 2mL collection tube. Add 500uL Buffer AW1. Centrifuge at >= 8,000 RPM for 1 min. Discard the flow through and collection tube.
6. Place column in a new 2mL collection tube. Add 500uL Buffer AW2. Centrifuge at 14,000 RPM for 3 min. Discard the flow through and collection tube.
7. Transfer the spin column to a new, labelled 1.5mL or 2mL tube.
8. Elute the DNA by adding 50uL Buffer AE to the center of the spin column membrane. Incubate at room temperature for 1 min. Centrifuge 1 min. at >= 8,000 RPM.
9. Repeat step 8, for a final elution volume of 100uL.

Completed Round 1 extractions at 3:00pm, July 11, 2025. Stored in fridge.

Completed Round 2 extractions at 10:00am, July 12, 2025. Stored in fridge.