FastQCFastQC Report
Mon 4 Mar 2024
RESUB-94_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRESUB-94_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16934870
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1194430.7053080419276913No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA300640.17752719684296364No Hit
TGAAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGAC170520.1006916498325644No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)