FastQCFastQC Report
Mon 4 Mar 2024
RESUB-94_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRESUB-94_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16934870
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG244560.14441209173734432TruSeq Adapter, Index 27 (97% over 38bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA222750.13153333919894278No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG209900.12394544510822936TruSeq Adapter, Index 27 (97% over 38bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT192440.11363535710637283No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG181110.10694501935946364No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)