FastQCFastQC Report
Mon 4 Mar 2024
RESUB-36_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRESUB-36_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15030980
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1491040.9919779016404786No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA263250.1751382810701631No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA205580.13677085592556174No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT197740.13155496181885679No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA190030.12642555575218647No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA155650.10355279562610023No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)