FastQCFastQC Report
Mon 4 Mar 2024
RESUB-116_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameRESUB-116_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15996427
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT246650.15419068270683198No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG242380.15152133660848138TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG239680.1498334596844658TruSeq Adapter, Index 27 (97% over 38bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA229730.14361332064966756No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG209020.13066667950286648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)