FastQCFastQC Report
Mon 4 Mar 2024
98_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename98_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22320535
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6950813.1140875431525274No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA661540.29638178475560734No Hit
GGAGATTTAGCATTTTAGGTCCGCCATCCATCGATAATACACAATGGCCG434450.1946413918841999No Hit
CGGACACGTACATATTCAACACGCATGTGCTTTATGTGCTATGAACAACT415400.18610665022142167No Hit
AGGAGATTTAGCATTTTAGGTCCGCCATCCATCGATAATACACAATGGCC308920.13840170049687428No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA293260.13138573963392902No Hit
CCTCACTTCATGCATGCATGCGGAAACTCATGCAAGCAAAGACATACACG273510.122537385416613No Hit
TTTCACTGGAAAATACCAACTTGAGTCCCAGGAGAACTTTGAGTCTTTCA264790.11863066902294234No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229670.10289627914384669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)