FastQCFastQC Report
Mon 4 Mar 2024
97_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename97_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18140324
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2640581.4556410348569297No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA570390.31443209062859073No Hit
TGTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAA357100.19685425684789312No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA313250.1726815904721437No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA267040.14720795505085799No Hit
GGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAAGAA252670.13928637658291No Hit
GTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAAT245320.13523462976736247No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241340.13304062264819527No Hit
TGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATA209960.11574214440712306No Hit
GTGTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAA206470.11381825374232567No Hit
CCTCACTTCATGCATGCATGCGGAAACTCATGCAAGCAAAGACATACACG203790.1123408821143437No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC193960.10692201528484276No Hit
GGAGATTTAGCATTTTAGGTCCGCCATCCATCGATAATACACAATGGCCG187460.1033388378289164No Hit
GTTGGGTTGACTTGGTGATGTTAATAACAAAAGATACATACCAAACCTAT185840.1024457997552855No Hit
CGGACACGTACATATTCAACACGCATGTGCTTTATGTGCTATGAACAACT183620.10122200683956914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)