FastQCFastQC Report
Mon 4 Mar 2024
97_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename97_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18140324
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGGGG638250.351840463268462TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT596690.328930177873339No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTGGGGG486590.26823666435064775TruSeq Adapter, Index 10 (97% over 37bp)
GTCCATTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGC429340.23667713983498861No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGTGG400190.2206079670903342TruSeq Adapter, Index 10 (97% over 37bp)
CAAAAATGAAGCCATAGCAGTAATCAATAATACCTTCTAGAGTCTGACTA359470.1981607384741309No Hit
CTTTATTCAAATAGATTAAATGAAAATACACTGTTACAATCCAGATTTTT336350.1854156518924359No Hit
GGAAAATCTGTTATACCAAAAATGAAGCCATAGCAGTAATCAATAATACC293150.16160130326227912No Hit
TTTTGCATCATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACT277300.1528638628505202No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT252330.13909894883906154No Hit
AAGAAATCGAAAGATTCGAAACACAAATCAAATTCATATTTGGATTGAAA237670.1310175055307722No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTGGTGG233640.12879593550809787TruSeq Adapter, Index 10 (97% over 37bp)
CACCAAGTCAACCCAACGAAATTAACCGGATACAATTTAATTTATACCAC228510.12596798160826675No Hit
TGCATCATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACTGTT224920.12398896513645512No Hit
TCATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACTGTTACAA214850.11843779637011997No Hit
CGGAAAATCTGTTATACCAAAAATGAAGCCATAGCAGTAATCAATAATAC201310.11097376210039028No Hit
TTTGCATCATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACTG194060.10697714109185702No Hit
CATGATTTGCCTTTATTCAAATAGATTAAATGAAAATACACTGTTACAAT190500.10501466236214965No Hit
TTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGCAGTAA183230.10100701619221356No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)