FastQCFastQC Report
Mon 4 Mar 2024
90_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename90_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18289278
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG970770.5307863984570632TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT753770.41213764698639277No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG677670.3705285687056646TruSeq Adapter, Index 10 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317510.17360444737074915No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC278450.15224767210602846No Hit
ATGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTC276600.151236150492108No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG228880.12514436053735964No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGTGG225510.12330175089470455TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGTGG200160.10944117094179442TruSeq Adapter, Index 10 (97% over 37bp)
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC185450.10139820719002686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)