FastQCFastQC Report
Mon 4 Mar 2024
88_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename88_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20182056
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG1204080.5966091859025661TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG959260.4753034081364158TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT859120.42568507390921917No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488800.24219534422062847No Hit
ATGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTC475040.23537740654371386No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC363420.18007085105699835No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGTGG305530.15138695482759537TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGTGG302530.1499004858573378TruSeq Adapter, Index 10 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG260970.12930793572270338No Hit
TGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCA249090.1234215186004835No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC215960.10700594627227275No Hit
GTGAGTATCCACATACAAGTCCAACACAACACATAATAAGTTGTTCATAG203370.10076773149375862No Hit
TTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACCTT203040.10060421990703029No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)