FastQCFastQC Report
Mon 4 Mar 2024
83_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename83_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16560429
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1344240.8117181022303226No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA388230.23443233264065802No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT269270.1625984447625119No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC242680.14654209742996394No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA203370.12280478965852877No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA199580.12051620160323143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)