FastQCFastQC Report
Mon 4 Mar 2024
83_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename83_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16560429
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT444750.2685618832700529No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG320850.19374498088183587TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG242460.14640925062992027TruSeq Adapter, Index 10 (97% over 37bp)
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC173870.10499124147085803No Hit
GTCCATTTCATAATCCGGAAAATCTGTTATACCAAAAATGAAGCCATAGC169080.10209880432445319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)