FastQCFastQC Report
Mon 4 Mar 2024
79_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename79_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18994723
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT401850.21155875766127255No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG282890.1489308372646445No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG267650.14090755627233942TruSeq Adapter, Index 10 (97% over 37bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA212260.11174682568416501No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG198720.10461853010438742TruSeq Adapter, Index 10 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)