FastQCFastQC Report
Mon 4 Mar 2024
78_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename78_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18296609
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3128051.709633735956209No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA554830.3032419832549299No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC293980.160674581830983No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT210320.11495026209501444No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA201230.10998212838236857No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA188770.10317212331530941No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA185490.1013794414035956No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)