FastQCFastQC Report
Mon 4 Mar 2024
78_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename78_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18296609
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG859080.4695296270472851TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT687200.37558872247857517No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG628600.34356092978759073TruSeq Adapter, Index 10 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG233910.1278433615759073No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC220200.12035016980468895No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGTGG204610.11182946523041509TruSeq Adapter, Index 10 (97% over 37bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA201120.10992200795240255No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGTGG189040.1033196916434078TruSeq Adapter, Index 10 (97% over 37bp)
GCACGATGTTCTTCTGCATAGGCGGCGTTGATTTGTTGCTGTTTTTGTAA187930.10271302185011441No Hit
GTTGATTTGTTGCTGTTTTTGTAACTAGACACACATTCTCAGCAGAAAAA184620.1009039434575008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)