FastQCFastQC Report
Mon 4 Mar 2024
70_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename70_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15325434
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGGGG896640.585066628455677TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGGGG644710.4206797667198201TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT268760.1753686062006466No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTGGTGG213300.13918039776230806TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGACTATCTAGTGG196420.1281660277940579TruSeq Adapter, Index 10 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174920.11413706130606154No Hit
TTTGGATCCAATCAGTTGTTTATTGAAATTATGCTTCATTAACAAATAAG158250.1032597184523453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)