FastQCFastQC Report
Mon 4 Mar 2024
5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19920216
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2244541.1267648905011873No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA669380.3360304928420455No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA324700.16300024055964052No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC290090.14562593096380078No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT242010.12148964649780905No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)