FastQCFastQC Report
Mon 4 Mar 2024
5_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename5_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19920216
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTGATGAATCTGGGGG1179550.5921371535328733TruSeq Adapter, Index 9 (97% over 36bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT695540.3491628805631425No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG268820.13494833590157856No Hit
ATGAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTC254220.12761909810616512No Hit
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA223380.112137338269826No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC216950.1089094616243117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)