FastQCFastQC Report
Mon 4 Mar 2024
58-S_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename58-S_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15714480
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG1447370.921042248932195TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG1284420.8173480764237825TruSeq Adapter, Index 27 (97% over 38bp)
CTCAGATGCAACTGTAAAACCTTGGGGTGCTTATTTTTAAAACTAGCCAA355270.22607811394331853No Hit
GTTTTTATTGACCATTGAAACGGACCACATGCATCAATGATGGCGGGATG191510.12186849326226512No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186020.11837490009214433No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATATGGGGG178310.11346859711552656TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)