FastQCFastQC Report
Mon 4 Mar 2024
4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20853504
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1986410.9525545443106348No Hit
TCCAAATCCAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAG328830.1576857299377601No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA247330.11860356897334856No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA223600.1072241864005205No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)