FastQCFastQC Report
Mon 4 Mar 2024
48_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename48_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20220755
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5903212.9193815957910574No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA342340.169301294635141No Hit
TTTCACTGGAAAATACCAACTTGAGTCCCAGGAGAACTTTGAGTCTTTCA325510.16097816327827522No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC311300.15395073032634043No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA259720.12844228615598183No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244920.12112307379224958No Hit
ACTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACA223870.1107129778289683No Hit
TGTGAACAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAA218700.10815619891542132No Hit
CTTGAGTCCCAGGAGAACTTTGAGTCTTTCATGAAGGCAATTGGTCTCCC217520.10757264009182645No Hit
CTTTCACTGGAAAATACCAACTTGAGTCCCAGGAGAACTTTGAGTCTTTC209690.10370038111831137No Hit
GGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAAGAA207100.10241951895465823No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)