FastQCFastQC Report
Mon 4 Mar 2024
41_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16333017
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12949747.928565800182538No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGTTTAAGCCATGCATGTATAAG246950.151196805832015No Hit
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT221860.13583528383029297No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAACTAATGTG193510.11847780480483185No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA188470.11539203075586095No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187180.1146022195409458No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC172640.10570000631236715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)