FastQCFastQC Report
Mon 4 Mar 2024
41_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename41_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16333017
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG10010026.128702370174476TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATATGGGGG590350.36144577575594267TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTAGGGG563520.3450189269992188TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCGGGGGG391610.23976586811854786TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGT264220.1617704800037862TruSeq Adapter, Index 2 (97% over 37bp)
CTTTTACTTCCTCTAGATAGTCAAGTTTGATCGTCTTCTCGGCACGCGCG247700.15165599839882613No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224190.13726184207118625No Hit
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT187110.11455936156804343No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC171620.10507550442150401No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)