FastQCFastQC Report
Mon 4 Mar 2024
40_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename40_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16717895
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG6298623.7675915538409592TruSeq Adapter, Index 2 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT406260.24300906304292497No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT389740.23312743619935405No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATATGGGGG386770.23135089674866366TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTAGGGG351480.2102417798413018TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCGGGGGG267000.15970910213277448TruSeq Adapter, Index 2 (97% over 37bp)
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC238170.1424641080710221No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGG183150.10955326612590881TruSeq Adapter, Index 2 (97% over 37bp)
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC174120.10415186840209249No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGT168420.1007423482442018TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)