FastQCFastQC Report
Mon 4 Mar 2024
31_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename31_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19015313
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1371450.7212345124163878No Hit
TCCAAATCCAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAG1213310.6380699597214098No Hit
CCAAATCCAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAGA437960.2303196376520334No Hit
TGAAGACTTCAGCGGTTGTTCTGGTGCTTGCGTTGGCCTTCGGCTTTGGA249690.13130996055652622No Hit
CAAATCCAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAGAT233920.12301664453275105No Hit
GAAGACTTCAGCGGTTGTTCTGGTGCTTGCGTTGGCCTTCGGCTTTGGAG200040.10519942532631464No Hit
CAAATTCTTCTTCTAGACAAGCAAGAGGATCAGTCCAGAAAGATGAAGAC199560.10494699719115852No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)