FastQCFastQC Report
Mon 4 Mar 2024
31_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename31_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19015313
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGATCTGAATCTGGGGG487620.25643543180172734TruSeq Adapter, Index 2 (97% over 37bp)
CAGCTTTATTGTGATGCGTGTCAATCCACCTCCTGCCTACAAATGGTACA216590.11390293706971849No Hit
GTCGGACTTGTCGCACATTTGGACGTGGATGTGGGACTGGATTCCAGGGT203970.1072661806829054No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)