FastQCFastQC Report
Mon 4 Mar 2024
20_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17220964
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATCTGGGGG3197621.8568182361916559TruSeq Adapter, Index 1 (97% over 36bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT280260.16274350262854043No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACCTACGATATGGGGG259670.1507871452492439TruSeq Adapter, Index 1 (97% over 36bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG224990.13064889979445982No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)