FastQCFastQC Report
Mon 4 Mar 2024
20-S_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename20-S_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16570250
Total Bases2.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTAGGGG172760.1042591391198081TruSeq Adapter, Index 27 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCCAGCATCTGGGGG170480.10288317919162356TruSeq Adapter, Index 27 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)