FastQCFastQC Report
Mon 4 Mar 2024
150_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename150_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27537784
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4963171.8023127786898174No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA482100.17506855308328367No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC390270.1417216432520496No Hit
GTTTTTTTGGTTTGTGTGCCTTTTGCATCATGCTATTTGGATAGTTTATA312100.11333519066022162No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)