FastQCFastQC Report
Mon 4 Mar 2024
150_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename150_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27537784
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTGGGGG2409180.8748634240140746TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTAGGGG1301480.4726160972139225TruSeq Adapter, Index 5 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT649460.2358432327016582No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC350880.12741766004120011No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC283870.10308382112373313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)