FastQCFastQC Report
Mon 4 Mar 2024
149_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename149_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96920347
Total Bases14.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63782276.5808957534995205No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA1207680.12460541438218335No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTAGAAGTGTAGATC988470.10198787257746818Illumina Single End PCR Primer 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)