FastQCFastQC Report
Mon 4 Mar 2024
149_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename149_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96920347
Total Bases14.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTGGGGG33662993.4732634624182683TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTAGGGG17877581.8445641759825726TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTGGGG2532650.2613125188253814TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATATGGGGG2033390.20980011555261974TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCGGGGGG1398650.14430922332541793TruSeq Adapter, Index 5 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTAGGG1241880.1281340851988489TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATATAGGGG1158940.11957654258088861TruSeq Adapter, Index 5 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)