FastQCFastQC Report
Mon 4 Mar 2024
148_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename148_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24830839
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTGGGGG1256730.5061166076587263TruSeq Adapter, Index 5 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAAGAATCTAGGGG754080.303686879045851TruSeq Adapter, Index 5 (97% over 37bp)
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG262030.10552603558824573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)