FastQCFastQC Report
Mon 4 Mar 2024
147_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename147_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8789658
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG889531.012018897663595TruSeq Adapter, Index 7 (97% over 38bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173180.19702700605643586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGT131570.14968728021044733TruSeq Adapter, Index 7 (97% over 38bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT105390.11990227606125291No Hit
CTCACACACAGACACTCACTCAAACACAAACCTTAATCAGTGCATCAAAC91840.10448643166776227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)