FastQCFastQC Report
Mon 4 Mar 2024
139_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename139_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9694605
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1159361.195881626946121No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA211640.21830698620521413No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT172560.1779959059703825No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA163080.16821727135865772No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC121580.12540995739382885No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA111380.11488864167235281No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)