FastQCFastQC Report
Mon 4 Mar 2024
138_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename138_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8479903
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1544791.821707158678584No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT170270.20079239113937977No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA159120.18764365582955372No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA134660.15879898626198907No Hit
CAAAGACCAGGCAACCGAATCTGTGATTTGCTTTTCAATCCAAATATGAA130690.15411732893642768No Hit
GGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAAGAA114150.1346123888445422No Hit
GGAGAAGACATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAG97570.11506027840176944No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)