FastQCFastQC Report
Mon 4 Mar 2024
137_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename137_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8306103
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGG990491.1924846104123679TruSeq Adapter, Index 7 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATGAGATCTGGGGT148780.17912130393759865TruSeq Adapter, Index 7 (97% over 38bp)
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGGTGCGTTCG102800.12376441756140033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGATGAGATCTGGGGG88440.10647592499153935TruSeq Adapter, Index 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)