FastQCFastQC Report
Mon 4 Mar 2024
129_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename129_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9630425
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167909117.43527414418367No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTAGAAGTGTAGATC265390.2755745462946858Illumina Single End PCR Primer 1 (97% over 37bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241530.2507989003600568No Hit
CATGGATAGAGTACTGGCCAACAGATCCAGAGTGTGCTGAAGATTTTCAA116030.1204827408966894No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTAGAAGTGTCGATC113390.11774142885698193Illumina Single End PCR Primer 1 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGGGGGGGG96600.10030709963475132No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)