FastQCFastQC Report
Mon 4 Mar 2024
121_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename121_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21456291
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3642121.6974601994352145No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA329950.15377774285406548No Hit
CCTCACCCGGCCCGGACACGGAAAGGATTGACAGATTGATAGCTCTTTCT320240.14925226359019833No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT235880.10993512345633268No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)