FastQCFastQC Report
Mon 4 Mar 2024
119_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename119_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22447450
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2183690.972800919480832No Hit
TGAAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGAC536960.23920757146134639No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA440410.19619600444593926No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT433090.19293505498397368No Hit
GGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGACTTCA299150.13326680758838977No Hit
AAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGACTT263840.11753673579849827No Hit
CTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAAAATTC228870.10195812887432648No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)