FastQCFastQC Report
Mon 4 Mar 2024
117_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename117_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22489339
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2620541.1652365594204437No Hit
TGAAGGCCTACCTGCTGTTACTGCTCCTGCTCCCGGTCTGCCTGGCTGAC326380.14512654195839192No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA318790.14175160950706467No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)