FastQCFastQC Report
Mon 4 Mar 2024
109_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename109_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22210202
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCTGGGGG2810851.2655670578772764TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATCTAGGGG578290.26037133746014557TruSeq Adapter, Index 7 (97% over 36bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT370910.1669998318790617No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGGCGTAATATGGGGG287080.1292559158174248TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)