FastQCFastQC Report
Mon 4 Mar 2024
108_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename108_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22634852
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGGGG1110870.49077855689093963TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTGGGGG881330.38936857197034025TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGTGG675010.29821710342970215TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTGGTGG413020.1824708197782782TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT405550.1791705993924767No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376670.16641151442032845No Hit
GTTTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACC342390.15126672796446824No Hit
TTTTTCTTTATTTTTCCTCACACACAGACACTCACTCAAACACAAACCTT257190.11362566011034665No Hit
TGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTG244290.10792648434370149No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)