FastQCFastQC Report
Mon 4 Mar 2024
100_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename100_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17927211
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1795221.0013939145358415No Hit
GGAAAATTCGCTTTGGCACTCATCCTCTGCCTCCAGGTCTCCCTGAGCCT259990.14502534722216412No Hit
TGTCACTTCTTGGTTCTGCACGTCTAACTCACCAGCAGAGATGAACGGAA240360.134075512359396No Hit
CTGTATGCCAGCATAGGCAGTGACCAGCGGCGCTTAGTGAAAACACACAC214080.11941623267556789No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA200350.11175748419539437No Hit
CCCACACATACACACACACACACACACACACACACACACACACACACACA180130.1004785406943668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)