FastQCFastQC Report
Mon 4 Mar 2024
100_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename100_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17927211
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGGGG382320.21326239759212962TruSeq Adapter, Index 10 (97% over 37bp)
GAAATATTCATATCTTTATTGAGCTGTATTTGATGTACAGTTTTCTTCAT330620.1844235559005804No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTGGGGG277280.15466990375692014TruSeq Adapter, Index 10 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAAGCGTTCATCTAGTGG244480.13637369471469935TruSeq Adapter, Index 10 (97% over 37bp)
GTGAGATCGAAAGCTATTGAGTCCCACCATTCTGTTGCAGAGAAAGTGCA205500.11463021213952355No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186530.104048532702605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)