FastQCFastQC Report
Wed 30 Apr 2025
2G2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2G2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10811600
Total Bases268.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG5960635.5131802878389875No Hit
TGAAAATCTTTGCTCTGAAGTGGAA3868143.5777683229124273No Hit
GTTGGATCCGGCCCGGACTGGCC3561233.2938972954974286No Hit
CTCGGGCTGAGACTTGAAGCGGTT3384583.130507972917977No Hit
CAGCTGTGACCCTCGGGTC2240132.071968996263273No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1853771.714612083317918No Hit
CGTGGTGGACTGCTTGCAGTGCT1587481.4683118132376336No Hit
TGAAAATCTTTGCTCTGAGGTGGAA1582411.4636224055643936No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1436751.3288967405379408No Hit
CGGACTGGCCGTGGTCGAGGC1035270.9575548484960598No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA1001540.9263568759480558No Hit
CTCGGGCTGAGACTTGAAGC997180.9223241703355655No Hit
GGATCCGGCCCGGACTGGCC858310.79387879684783No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA683840.6325058270746236No Hit
TCTCGGGCTGAGACTTGAAGCGGTT681840.6306559621147656No Hit
CTTTCCGAGGTCGCGGTC644730.5963317177846017No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC595580.5508712863960932No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT585310.5413722298272227No Hit
TCTCGGGCTGAGACTTGAAGCG557100.5152798845684264No Hit
CTCGGGCTGAGACTTGAAGCA516750.47795885900329277No Hit
GAGTGTGCTCTTAACTGA506960.4689037700247882No Hit
GATCCGGCCCGGACTGGCC489220.4524954678308484No Hit
TTGGATCCGGCCCGGACTGGCC483940.4476118243368234No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT435650.40294683488105365No Hit
CGAGTGTGCTCTTAACTG399500.3695105257316216No Hit
GTTGGATCCGGCCCGGACTGGCT390620.36129712530985236No Hit
TCCCTTTCCGAGGTCGCGGTC323170.29891042953864366No Hit
GTTGGATCCGGCCCGGACTGGC301830.2791723704169596No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC296900.2746124532909097No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC289570.26783269821303046No Hit
CTCGGGCTGAGACTTGAAGCGG275040.2543934292796626No Hit
CAGCTGTGACCCTCGGGT267740.24764142217618113No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA252670.23370268970365166No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC240300.22226127492693035No Hit
CTCGGGCTGAGACTTGAAGCGGT237120.21931998964075622No Hit
GAGGTGCCGTGGAAGGTAGGTCT233750.21620296718339563No Hit
CTCCCTTTCCGAGGTCGCGGTC227020.20997817159347365No Hit
CCCTTTCCGAGGTCGCGGTC221960.20529801324503313No Hit
TGGATCCGGCCCGGACTGGCC217300.20098782788856412No Hit
GGAAGGAATCGGCCGTGGATTGGCC206770.19124828887491213No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT203850.18854748603351956No Hit
GGACTGGCCGTGGTCGAGGC202680.18746531503200267No Hit
GAGTGTGCTCTTAACTG193610.17907617743904697No Hit
GAGACCGAGTGTGCTCTTAACTG188750.17458100558659218No Hit
CGACTGCTGCCGGTCGTTGGGGCG178510.16510969699211958No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT174920.1617891893891746No Hit
TGAAAATCTTTCTTCTGAGGCGGAA171320.15845943246143032No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC168830.1561563505864072No Hit
CTCGGGCTGAGACTTGAAGCGGTC166340.15385326871138408No Hit
CTCGGGCTGAGACTTGAAGCGGTTG163940.15163343075955454No Hit
CGACCAAGATAGTCGAACTCGACCCTGT160220.1481926819342188No Hit
TTCTGCTGAACTACTTTGAGAACGAGT158670.1467590365903289No Hit
GGATCCGGCCCGGACTGGC156580.14482592770727737No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA155850.1441507269969292No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG150890.13956306189648154No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT147290.13623330496873728No Hit
GTTGGATCCGGCCCGGACT145100.13420770283769284No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC144990.13410596026490065No Hit
CTTTCCGAGGTCGCGGTCGGA144190.13336601428095748No Hit
GTTGGATCCGGCCCGGACTGGCA141490.1308686965851493No Hit
GTTCTTCCTCGCCGAGACCGAGTGTGCT140800.1302304931739983No Hit
CGGGCTGAGACTTGAAGCGGTT139340.128880091753302No Hit
CTACCGATAGCCAACTCGAACGTTTCCG133590.12356172999371046No Hit
GTTCTATTTTGTTGGTTTCTGGAACC131640.12175811165784899No Hit
TTGTCTACAAGAAGCCTGTCCATTTTGT129170.11947352843242444No Hit
CGTGGTGGACTGCTTGCAGTGCC128740.11907580746605498No Hit
GAGTGTGCTCTTAACT127630.11804913241333383No Hit
CGTGGTGGACTGCTTGCAGT124170.11484886603277962No Hit
CGGACTGGCCGTGGTCGAGGT124140.11482111805838173No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC122530.1133319767656961No Hit
CGACTGCTGCCGGTCGTTGGGGC122000.11284176255133375No Hit
GACCGTGGTGGACTGCTTGCAGTGCT121890.11274001997854156No Hit
GAGACTTGAAGCGGTTGGAT120500.1114543638314403No Hit
CGTAGTTGGATTTCGGGATGGCCCG116040.10732916497095711No Hit
CGTGGTGGACTGCTTGCAGTGCTC114400.10581227570387362No Hit
GAGACCGAGTGTGCTCTTAACTGA110590.10228828295534426No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA109050.10086388693625364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)